Potassium solubilization test

• microbial cultures are grown for 48h in rich liquid media.
• 5 uL of culture is placed onto Aleksandrow Agar.
  • NOTE: If multiple isolates are tested on the sample plate inoculation must be a minimum of 1 cm apart.
• Plates are then incubated at 22C for 7 days
• A positive phenotype is a zone of clearing around the colonies.
• At 7 days, the ability of each colony to solubilize phosphate is recorded.
  • If a colony is positive for K-sol then the zone diameter will be measured.
    • The clearing is measured at two perpendicular right angles.