Tryptic soy agar

 TSA is generally purchased as a premade dehydrated media.

Direction of making in lab:

• Add 1000 mL of nano-pure H2O a 2 L flask
• To the flask add:
  • 15 g Casein peptone
  • 5 g soy peptone
  • 5 g NaCl
  • 15 g agar
• Autoclave at 121 °C and 15 psi for 30 min.

Note: for broth media, omit addition of agar