Starch casein agar (SCA)

For the isolation of Actinomycetes and starch degrading microbes

Step 1:

• Prepare mineral and trace metal solutions A and B

SCA agar recipe

1. 400 mL DI-H2O
2. 5.0 g starch
3. 0.15 g casein
4. 0.01 g CaCO3
5. 50 mL Solution A
6. 50 mL Solution B
7. 7.5 g agar
8. Autoclave at 121 °C and 15 psi for 30 min.
   • use within 24 hrs
9. Pour plates (unless making broth, then don't do that, it will be a mess)