R2A medium

 R2A is generally purchased as a premade dehydrated media.

Recipe for R2A medium

• 0.5 g yeast extract
• 0.5 g protease peptone (Difco no. 3)
• 0.5 g casamino acids
• 0.5 g glucose
• 0.5 g starch
• 0.3 g pyruvate
• 0.3 g K2HPO4
• 0.05 g MgSO4 x 7-H2O
• 15.0 g agar
• 1000 mL nano-pure H2O
• Autoclave at 121 °C and 15 psi for 30 min.