Lipase agar

Defined media

• 0.5g peptone
• 0.3g yeast extract 0.3%
• 0.5g Na2HPO4 (SUBSTITUE: K2HPO4)
• 0.5g MgSO4 (magnesium sulfate)
• 0.02g CaCO3 (calcium carbonate)
• 0.01g FeSO4 (ferric sulfate)
• lipid source (one of the following)
  • 0.1% tributyrin
  • 0.1% tween80
  • 0.1% cooking oil
• 15g agar
• 1000ml DI-H~2~O

undefined media

• Premade rich media base
  • 15g agar (in needed)
• lipid source (one of the following)
  • 0.1% tributyrin
  • 0.1% tween80
  • 0.1% cooking oil
• 1000ml DI-H~2~O