King's B medium

 King's B can be purchased as a premade dehydrated media. For Pseudomonad modification see "King's B (pseudomonads)" below.

King's medium B (premade)

• 20.0 g Proteose peptone no. 3
• 1.5 g K2HPO4
• 1.5 g MgSO4 x 7-H2O
• 15.0 g agar
• 1000 mL nano-pure H2O
• Autoclave at 121 °C and 15 psi for 30 min.

King's B (pseudomonads)

• 10.0 g Tryptone [^1]
• 10.0 g Proteose peptone no. 3 [^2]
• 1.5 g K2HPO4
• 1.5 g MgSO4 x 7-H2O
• 10 mL glycerol [^1]
• 15.0 g agar
• 1000 mL nano-pure H2O
• Autoclave at 121 °C and 15 psi for 30 min.

[^1]: These items are not present in the premade dehydrated King's medium B. [^2]: Proteose peptone is half the amount compared to King's medium B